SPE Extraction and TLC Identification of Tetracycline and Fluoroquinone in Surface Water

Abstract

Simultaneous identification of tetracycline, oxytetracycline, chlortetracycline, ciprofloxacin, and enrofloxacin in surface water employed solid-phase extraction and thin-layer chromatography separation on TLC silica gel 60 F254 plates. Mobile phase: water/methanol/dichloromethane (6/35/59). Plates were impregnated with 10% EDTA solution pH 9.0. Visualization occurred under UV light at λ=254 nm and λ=366 nm. Method detects antibiotics at concentrations ≥5 μg/ml.