Optimization and Validation of Europium-Sensitized Fluorescence Method for Determination of Tetracycline Antibiotics in Water from Fish Farms

Abstract

A europium-sensitized fluorescence technique was developed and validated for detecting tetracycline antibiotic residues in fish farm water. The method is highly sensitive and particularly applicable to samples where low concentrations of tetracycline antibiotics are expected. Detection limits ranged from 0.65–1.29 µg/L across three tetracycline variants tested.