Utilisation of Derivative UV/Vis Spectrophotometry in Determination of Active Pharmaceutical Ingredient Content in Some Drugs

Abstract

The aim of this paper was to investigate the utilization of derivative UV/Vis spectrophotometry in the quantification of active pharmaceutical ingredients (API) in some drugs. Meloxicam, naproxen and acetylsalicylic acid (ASA) were analyzed in different drug samples using a Perkin-Elmer Lambda 25 spectrophotometer. A third-order derivation was made using computer software after recording the absorption spectra. The obtained API content was within acceptable limits for naproxen in Nalgesin S and meloxicam in Melox, with observed deviations of less than 10% of the declared values, in line with U.S. Pharmacopoeia requirements. Based on the results, UV/Vis derivative spectrophotometry is concluded to be a suitable method for determining API content in some drugs.