Phytochemical screening, quantitative determination of phenolic compounds, and antioxidative activity of Ostrya carpinifolia

Abstract

Ostrya carpinifolia is an interesting and suitable species for reforestation and landscaping. For the first time ever, phenolic profile, antioxidative and antimicrobial activity of O. carpinifolia was done in this study. Aqueous and methanol extracts of the aerial parts were analyzed using fast screening methods of secondary metabolites, and UV/VIS spectrophotometry for determination of polyphenolic contents and antioxidant activity (DPPH). Phytochemical tests confirm the presence of cardiac glycosides, coumarins, emodins, flavonoids, tannins, terpenes, terpenoids and steroids. Methanol extracts of leaves contain the highest level of total phenolics and flavonoids (35.574 and 30.908 mg CE g-1 DW, respectively), while the inflorescences extracts were the richest with total proanthocyanidins and phenolic acids (19.165 mg CE and 9.342 mg CAE g-1 DW, respectively). All methanol extracts showed very strong antioxidative activity, where the lowest activity was recorded for inflorescences (IC50: 0.242 mg mL-1) and the highest for stem (IC50: 0.107 mg mL-1). No antimicrobial activity was detected for any of the tested organisms.