Determination of phenolic content and antioxidant properties of methanolic extracts from Viscum album ssp. album Beck.

Abstract

Content of phenolic compounds and antioxidant properties of methanolic extracts from Viscum album ssp. album Beck. leaves and stems was determined. Mistletoe was collected from four different hosts (Betula L., Tilia cordata Mill., Robina pseudoacacia L, and Salix alba L.). Folin-Ciocalteu method, AlCl3 method, method with Arnow reagent, and acid-butanol assay were used for determination of total phenols, flavonoids, phenolic acids and proanthocyanidins respectively. Antioxidant properties of the extracts were investigated with DPPH, ABTS and FRAP method. Total phenols were in range 7.02-13.52 mg GAE/g DW, flavonoids 2.29-5.05 mg RE/g DW, phenolic acids 0.62–2.84 mg caffeic CAE/g DW and proanthocyanidins 0.63 -4.83 mg LCE/g DW. Content of flavonoids and proanthocyanidins was higher in leaves than the stems. The highest antioxidant activity in leaves (68.93-86.89 μmol Trolox equivalents/g DW) and in stems (67.28-81.72 μmol Trolox equivalents/g DW) with DPPH, ABTS and FRAP method had mistletoe collected from Robina pseudoacacia L. Also, high correlation was obtained between total phenols, phenolic acids and proanthocyanidins content and antioxidant activity.